The Affinity and Stoichiometry of Binding of Human Factor VI11 to von Willebrand Factor

Relative Stoichiometry and Affinity for Describing Von Willebrand Factor Binding of Coagulation Factor VIII

Effect of the Multimeric Structure of the Factor Vill/von Willebrand Factor Protein on Binding to Platelets

The characteristics of the intact factor VIll/von Willebrand factor protein binding to human platelets was compared to 2-mercaptoethanol-treated factor VIll/von Willebrand factor protein and to fractions of plasma factor VIll/von Willebrand factor protein that elute after the void volume. These studies indicate that the factor VIlI/von Willebrand factor protein larger size oligomers bind prefer...

Frequency Assessment of the H817Q (2451T→A) Variant of von Willebrand Gene in Individuals without Hemorrhagic Signs

Abstract Background and Aims:‎ Von Willebrand disease is a bleeding disorder caused by quantitative or functional defects in von Willebrand factor. The disease is found in up to 1 percent of the population. The most common symptom is mucocutaneous bleeding. Recently, studies conducted on healthy people showed that the H817Q mutation that previously known to cause von Willebrand...

The affinity and stoichiometry of binding of human factor VIII to von Willebrand factor.

To study the interaction between factor VIII and von Willebrand factor (vWF), binding experiments were performed using immobilized plasma vWF. Plasma was obtained from healthy donors and from patients with severe hemophilia A. For normal and hemophilic vWF, the dissociation constants (kd) for binding of factor VIII to vWF were 0.21 +/- 0.04 and 0.22 +/- 0.05 nmol/L, respectively. At saturation,...

Effect of multimeric structure of the factor VIII/von Willebrand factor protein on binding to platelets.

The characteristics of the intact factor VIII/von Willebrand factor protein binding to human platelets was compared to 2-mercaptoethanol-treated factor VIII/von Willebrand factor protein and to fractions of plasma factor VIII/von Willebrand factor protein that elute after the void volume. These studies indicate that the factor VIII/von Willebrand factor protein larger size oligomers bind prefer...